Polyhydroxybutyrate production from dark-fermentative effluent and composite grafting with bagasse derived α-cellulose in a biorefinery approach

The study evaluated the preparation of a biocomposite using waste-derived polyhydroxybutyrate (PHB) and bagasse cellulose (α-cellulose) in biorefinery approach. PHB was produced dark fermentation effluent rich volatile fatty acids (VFA) derived from vegetable waste α-cellulose extracted sugarcane (SCB). Nutrient limitation induced microbial accumulation, wherein maximum production 0.28 ± 0.06 g PHB/g DCW (28%) observed. Confocal examination showed deposition granules cell cytoplasm NMR spectrum exhibited structural correlation. α-Cellulose (0.22 0.02 α-cellulose/g SCB) through SCB pretreatment. Thereafter, grafting with offered intermolecular bonding, which resulted enhanced thermal stability than corresponding pristine PHB. FE-SEM morphological depicted that functioned as filler to XRD profiles significant decrement crystallinity, signifying functional role an effective reinforcing agent. Additionally, ether group ester also appeared XPS analysis composite, thus authorizing blending Utilization bagasse-derived for strengthening biologically expands its applications, while simultaneously addressing plastic pollution issues. Additional value this process further achieved by incorporating concept biorefinery, acidogenic effluents were used PHA, enabled re-entry products cycle, achieving circularity.